Ctcf isoform
WebAlthough the open reading frame of the major isoform of CTCF would only encode an 82 kDa protein, CTCF migrates at a much higher molecular weight ( 130 kDa) (41). In our studies, we observed the ... WebMar 11, 2014 · CCCTC-binding factor (CTCF) is a DNA-binding protein that has various, often seemingly contradictory, roles in gene regulation. This Review describes these …
Ctcf isoform
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WebFeb 28, 2005 · A control pSG5 plasmid that does not express CTCF was also exploited to establish the basal focus-forming potential of cells in the absence of any exogenous CTCF isoform. Twenty-four hours post-transfection, cells were subjected to G418 selection and two weeks later the number of colonies was counted. WebHere, we explore the functions of an alternatively spliced human CTCF isoform in which exons 3 and 4 are skipped, producing a shorter isoform (CTCF-s). Functionally, we find …
WebCTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. WebTopologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins. ... article] [Google Scholar] 12. Revenkova E, Eijpe M, Heyting C, Gross B, Jessberger R. Novel meiosis-specific isoform of mammalian SMC1. Mol Cell Biol 2001; 21: 6984–6998. [PMC free article] [Google …
WebApr 4, 2024 · Identification of an alternatively spliced CTCF-s isoform in the human genome. a Schematic representation of exons of the human CTCF gene. Transcripts including alternative exons 3 and 4 encoded ... WebNational Center for Biotechnology Information
WebApr 12, 2024 · Putative motifs of CTCF’s zinc fingers identified by interpreting wild-type versus mutant differential peak prediction models. To identify motifs related to the binding of each ZF in CTCF, we trained and interpreted a neural network for predicting whether a peak would be significantly weaker according to DESeq2 [] in the mutant dataset than in the …
WebNov 3, 2011 · To dissect the impact of CTCF on exon 5 splicing in a cell-based system, we screened several human Burkitt lymphoma B cell lines for differences in expression of the exon 5 containing CD45RB isoform. grapeseed unit 2 pdfTranscriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, V(D)J recombination and regulation of chromatin architecture. grapeseed unit 1 helloWebNov 30, 2010 · CTCF paralog; CTCF-like protein; Cancer/testis antigen 27 (CT27) Zinc finger protein CTCF-T; Gene names. Name. CTCFL. Synonyms. BORIS. Organism … chippy and the ya yas bandWebJun 19, 2014 · It is closer to that of the ENSEMBL ENST00000369429 isoform than to the RefSeq isoform and ~1.6 kb from the 5' end of the RefSeq isoform (Figure 3a). Moreover, the position of the active promoter-type chromatin seen specifically in Mb ( Figure 4 b), which overlaps a constitutively unmethylated DNA region, is consistent with the use of … chippy and snowflakeWebThe short isoform CCAT1-S is also referred as CCAT 1 or cancer-associated region long noncoding RNA-5 (CARLo-5), it is named as CCAT1-S after the long isoform CCAT1-L is discovered . ... (CTCF), regulating the intra-chromosomal interaction of well-studied oncoproteins c-MYC causing CRC tumorigenesis. chippy anaheimWeb5.1 CTCF genome organizer and the cohesin complex. CTCF (CCCTC-binding factor) is a multiple zinc finger protein that exerts diversified functions under different genomic … grapeseed unit 3 pdfWebAug 1, 2003 · The major isoform of CTCF has a molecular mass of 82 kDa [8]. However due to amino acid sequences in the N- and C-termini, CTCF migrates aberrantly in SDS–PAGE gels [8]. Full-length CTCF migrates at approximately 130 kDa, although several reports in the literature observe a band of approximately 140 kDa [5], [8], [9]. chippy and noppo