WebWith its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar … With its nonpolar structure and long-wavelength absorption and … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebInvitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza- s -Indacene) Catalog number: D3922 Related applications: Cellular Imaging Technical …
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WebJan 16, 2024 · Briefly, 2D or 3D 3T3-L1 cells were incubated in a mixture of 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor 594 phalloidin (# 20553, Funakoshi, Tokyo, Japan), and DAPI (# D523, Dojindo, Kumamoto, Japan), at 1:1000 dilutions in PBS for 1 hr, and thereafter fixed in 4% paraformaldehyde in PBS for … WebJul 4, 2024 · They were cultured in Dulbecco’s Modified Eagle’s medium (Thermo Fisher Scientific) media containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) in an incubator at 28℃. ... (D3922; Thermo Fisher Scientific) for 30 min, followed by thrice PBS washes. The IECs were observed using a laser scanning confocal microscope …
WebIn order to identify podocyte proteins that could perform as a similar or better biomarker for the presence of CPF (s), or are involved in the mechanism of action of CPF (s), we here followed a proteomics approach by analyzing proteomes of hPod exposed to presumably CPF-containing plasmas from patients with FSGS. WebFeb 9, 2024 · To visualize the accumulation of lipids in response to OA treatment, we used boron-dipyrromethene (BODIPY) 493/503 (D3922, Thermo Fisher Scientific, MA, USA), which labels specifically ...
WebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, … WebJan 1, 2024 · Shikonin was purchased from Selleck (S8279). Lipofectamine 3000 for siRNA transfection (Invitrogen), HilyMax for plasmid transfection (Dojindo, Kumamoto, Japan). Puromycin（Sigma，St. Louis, MO, USA). BODIPY 493/503 …
WebOct 21, 2024 · LipidSearch™ (Thermo Fisher Scientific) was used to make lipid peak assignments in the single cells. Only peaks above a 1E4 intensity threshold and within 5 ppm of the calculated m / z value were selected. ... (#D3922, Invitrogen, USA) in PBS and incubated in the dark for 15 minutes at 37 °C with 5% CO 2. After incubation, cells were …
WebThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. ct filing datesWebNov 18, 2024 · DFCP1 is an ER-associated protein with two PI (3)P-binding FYVE domains. In WT cells, myc-DFCP1 formed very small punctate structures ( Figure 1E) ( Axe et al., 2008; Li et al., 2024 ). In SKO cells, the myc-DFCP1 structures appeared highly irregular and significantly enlarged ( Figure 1, E and F). ct filing instructionsWebIECs were cultured in 12-well plates for 36 h, then washed twice with PBS and incubated with the 5 g mL −1 Bodipy 493/503 (D3922; Thermo Fisher Scientific, Waltham, MA, USA) for 30 min, followed by three PBS washes. A laser scanning confocal microscope (Leica DMI8) was used to visualize the fluorescence intensity in the IECs. ct filing extensionWebFeb 24, 2024 · Finally, BODIPY493/503 (#D3922, Thermo Fisher Scientific, USA) staining was performed to check the lipid droplet formation. ... USA) according to the manufacturer’s instruction. SYBR Green qPCR Master Mix (#K0251, Thermo Fisher Scientific, USA) was used for quantitative real-time PCR amplification with a CFX96 real-time PCR detection … ct filing uiWebAug 1, 2024 · For BODIPY lipid staining [6–8], 4% paraformaldehyde fixed 3D sphenoids were incubated in 0.1% BODIPY (# D3922, Thermo Fisher Scientific), 0.1% DAPI (#D523, Doujin, Japan) and 0.1% phalloidin (#20,553, Funakoshi, Japan) in phosphate buffered saline containing 3% bovine serum albumin for 3 hrs. ctf imagesWebSep 1, 2024 · Briefly, HepG2 cells were fixed with 4% paraformaldehyde for 10 min, then washed three times with phosphate buffer saline (PBS). After washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific, MA, USA) at room temperature for 1 h, followed by three washes with PBS. earth day night map liveWebJan 9, 2024 · The BODIPY 493/503 (D3922; Thermo Fisher Scientific, USA) staining was performed for primary IECs, as described by Wei et al. . The laser scanning confocal microscope (Leica Microsystems, Germany) … ctfinancy