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Glut3 molecular weight

Web1-氧杂-8-氮杂-螺[4,5]癸-3-酮1-Oxa-8-aza-spiro[4.155.19432133382-42-... Glucose transporter 3 (or GLUT3), also known as solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3) is a protein that in humans is encoded by the SLC2A3 gene. GLUT3 facilitates the transport of glucose across the plasma membranes of mammalian cells. GLUT3 is most known for its … See more GLUT3 was the third glucose transporter to be discovered, first cloned in 1988 from a fetal skeletal muscle cell line, using a GLUT1 cDNA probe and shown to share 64.4% identity with GLUT1. See more Click on genes, proteins and metabolites below to link to respective articles. See more Although GLUT3 was found to be expressed in various tissues, it is most specifically expressed in neurons, found predominantly in axons and dendrites and also, but less … See more • Glucose+Transporter+Type+3 at the U.S. National Library of Medicine Medical Subject Headings (MeSH) See more

Molecular basis for inhibiting human glucose transporters by

WebGLUT3 is one of fourteen facilitative sugar transporters, which use the glucose diffusion gradient to move across various plasma membranes to display various specificities, ... WebDec 20, 2014 · We noticed that Glut3 signals occurred as high molecular weight smears when samples were boiled, and therefore we incubated samples at room temperature for 30 minutes. Protein blotting was performed using the iBlot system (Invitrogen). firewall bbc bitesize https://amaaradesigns.com

Molecular basis for inhibiting human glucose transporters by

WebGlut3 Antibody (M-20) has been replaced by a more specific monoclonal antibody, Glut3 (G-5) that provides a stronger signal & more reproducible data. ... 5 by JM from Well defined band observed at expected molecular Well defined band observed at expected molecular weight by Western Blot using EOC 20 whole cell lysate. -SCBT QC. Date published ... WebJan 26, 2024 · The identified GLUT3 inhibitors had IC 50 values between ~ 7 and 38 µM for GLUT3 (Fig. 2, Table 2), and between ~ 6 and 25 µM in GLUT3 S66Y (Supplementary Fig. S3, Supplementary Table S2). WebJun 1, 2024 · GLUT3 expression is reported in monocytes and T lymphocytes in the human immune system , and in activated T lymphocytes in ... As previously reported we noted that GLUT3 protein aggregates to form high molecular weight smears in Western blot of boiled lysate and that copy number dependent protein levels at 54 kDa could only be observed … etsu pediatrics phone number

Glucose transporter 3 is a rab11-dependent trafficking cargo and …

Category:Glucose transporters in brain in health and disease

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Glut3 molecular weight

The glucose transporter GLUT3 controls T helper 17 cell responses ...

WebCalculated molecular weight: 492 aa, 54 kDa: Observed molecular weight: 45-55 kDa: GenBank accession number: BC121804: Gene symbol: SLC2A1: Gene ID (NCBI) 6513: Conjugate: Unconjugated: Form: Liquid: … WebOct 31, 2024 · We created a neural-specific conditional murine glut3 ( Slc2A3 ) deletion ( glut3 flox/flox/nestin-Cre+) to examine the effect of a lack of Glut3 on neurodevelopment. Compared with age-matched glut3 flox/flox = WT and heterozygotes ( glut3 flox/+/nestin-Cre+), we found that a >90% reduction in male and female brain Glut3 occurred by …

Glut3 molecular weight

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WebNOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) ... Rabbit mAb recognizes endogenous levels of total Glut1 … WebNational Center for Biotechnology Information

WebMay 1, 2024 · The gene quantification was performed using the following commercially available probes from Thermo Fisher Scientific: Rpl30 (Mm01611464_ g1, used for normalization), Glut1 ( Slc2a1) (Mm00441480_m1), Glut3 ( Slc2a3) (Mm00441483_m1), Arg1 (Mm00475988_m1), Hk2 (Mm00443385_ m1), Siglecf ( Siglec5) … WebDec 15, 2009 · Glut3. Glut3 (see Ref. 36) is the major neuronal glucose transporter, ... but Glut7 is most likely involved in the dietary absorption of one or more low-molecular …

WebJul 15, 2015 · The crystals of GLUT3 in the presence of d-glucose were obtained in the P2 1 Fig. 1c, Extended Data Fig. 2a–d and Extended Data Table 1). The transmembrane … WebApr 5, 2024 · GLUT3 controls the effector function of Th17 cells. To investigate the function of GLUT3 in T cells, we generated mice with T cell-specific inactivation of the Slc2a3 gene by crossing Slc2a3 fl/fl mice (Fidler et al., 2024) with Cd4 Cre animals. We first verified that GLUT3 expression was abolished in CD4 + T cells without a compensatory upregulation …

WebGLUT3 was first isolated from human fetal skeletal muscle.70 Human GLUT3 has 64% and 52% identity with human GLUT1 and GLUT2, respectively, with an 83% amino acid …

WebGlutor is a potent inhibitor of glucose uptake that targets glucose transporters GLUT1, -2, and -3. Inhibition of glucose uptake by glutor leads to upregulation of GLUT3 and GLUT1 … fire wall behind wood stoveWebMar 21, 2024 · We found that the glucose transporter GLUT3 is essential for the effector functions of Th17 cells in models of autoimmune colitis and encephalomyelitis. At the molecular level, we show that GLUT3-dependent glucose uptake controls a metabolic-transcriptional circuit that regulates the pathogenicity of Th17 cells. etsu pediatrics elizabethtonWebImmunoblots of brain membrane preparations showed that both antisera identified peptide-inhibitable protein bands of molecular weight 45,000-50,000. Immunocytochemical studies demonstrated that binding sites for these antisera were abundantly distributed in neuropil in all brain regions. etsu pediatrics johnson city tn phone numberWebJan 9, 2024 · Glucose Transporter Glut3 Antibody (G-5) is an IgG1 κ mouse monoclonal Glut3 antibody that detects m, r, and h Glut3 by WB, IP, IF and ELISA. 39 citations ... firewall basic conceptsWebThe molecular weight of GLUT3 transporter is 49-52 kDa and depends on the glycosylation pattern, which is tissue-specific (PMID: 9253355 and … firewall benefitsWebIn this study, we have demonstrated that in rat brain, GLUT3 is found exclusively in the neuronal/glial fraction and is absent from cerebral microvessels, whereas GLUTl is detected as different molecular weight forms in each fraction. firewall benchmarkWebJul 15, 2015 · The crystals of GLUT3 in the presence of d-glucose were obtained in the P2 1 Fig. 1c, Extended Data Fig. 2a–d and Extended Data Table 1). The transmembrane region of GLUT3 exhibits a canonical ... firewall before or after router cisco