Mcherry-gfp-lc3b

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August undefined, 2024

Web(F and G) GFP-LC3B-expressing HeLa cells were infected with mCherry-expressing Salmonellae. Correlative cryo-ET captures early phagophores (F) and expanded phagophores (G) around a damaged SCV. http://www.biofeng.com/adjy/zhili/pBABE-puro%20mCherry-EGFP-LC3B.html

Addgene: pDEST-CMV mCherry-GFP-LC3B WT

WebAd-mCherry-GFP-LC3B 关键词：Ad-mCherry-GFP-LC3B,YT160,百奥莱博 ·Tubulin抗体编号：YT156 英文名称：Tubulin antibody 规格：>40次本Tubulin抗体为进口分装，用纯化的微管蛋白作为抗原制备而成的抗Tubulin小鼠单克隆抗体。克隆号为B-5-1-2。本Tubulin抗体识别α-tubulin的C-terminal。 Web22 jul. 2024 · Amplify a fused eGFP-LC3B ORF from the pBabe-mCherry-eGFP-LC3B template and insert it into the pBob-eGFP vector, replacing the existing eGFP ORF: 1.1.1. define atoms and molecules

Antagonizing apolipoprotein J chaperone promotes proteasomal ...

Web14 okt. 2024 · Ad-mCherry-GFP-LC3B was used to demonstrate blockage of the autophagic flux in eukaryocytes . Infection of Ad-mCherry-GFP-LC3B resulted in overexpression of tandem mRFP/mCherry-GFP proteins. In cells lacking an autophagic state, mCherry-GFP-LC3B was present in the cytoplasm as diffuse yellow fluorescence … Web25 sep. 2024 · (F) Images of mCherry-LC3B and GFP-ATG4B in the starved cells coexpressing mCherry-LC3B and GFP-ATG4B or GFP-ATG4B LIRCM. Scale bar, 10 µm. To confirm that the membrane binding of ATG4B is dependent on LC3, we observed the distribution of ATG4B LIRCM , an ATG4B mutation that fails to interact with LC3, in cells … WebPlasmid EGFP-LC3 from Dr. Karla Kirkegaard's lab contains the insert LC3B and is published in PLoS Biol. 2005 May . 3(5):e156. This plasmid is available through … feed the world bag

顺铂通过增加HeLa人宫颈癌细胞中Nrf2入核促进细胞自噬和凋亡

WebGreen fluorescent protein (GFP)-LC3B adenovirus, GFP-red fluorescent protein (RFP)-LC3B adenovirus and mCherry-EGFP-LC3 adeno-associated virus were obtained from Hanbio (Shanghai, China). Cell Culture. The human HSC cell line LX-2 (Procell, Wuhan, China) was cultured in DMEM supplemented with 10% FBS at 37 °C and 5% CO 2. WebTHP1-Difluo™ hLC3 reporter cells are designed to monitor the autophagic flux by means of a fluorescent-labeled LC3B protein. These cells, derived from the THP-1 human monocytic cell line, express the RFP::GFP::LC3 fusion protein in which the human LC3B (microtubule-associated protein 1 light chain 3 beta) is fused to two fluorescent reporter ... define at one withWebBeyotime adenovirus expressing mcherry gfp lc3b fusion protein recognizing cd46 Adenovirus Expressing Mcherry Gfp Lc3b Fusion Protein Recognizing Cd46, supplied by Beyotime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more feed the world 2022

"Web7 nov. 2015 · Plasmids construction pQCXIP-tfLC3B expressing mCherry-EGFP tandem ﬂuorescent- tagged LC3B follows.First, mCherry gene ampliﬁedfrom pmCherry-C1 (Clontech, CA, USA) tem-plate PCR(up primer: -GTCGCGGCCGCCACCATGGTGAGCAA-GGGCGAGGAG-3 downprimer: -GCTGGATCCACCTCCGCTTCCA … " - Mcherry-gfp-lc3b

Mcherry-gfp-lc3b

Establishment of a cell line with stable expression of mCherry-EGFP ...

http://www.damor.cn/question/3143 Web7 apr. 2024 · LC3B-eGFP-mCherry assay by confocal and flow cytometry. LC3B-eGFP-mCherry L6 cells were plated on 6-well plates and cultured to reach 60% confluency for confocal and 80% confluency for flow cytometry.

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Web11 mei 2024 · mCherry (红光)-eGFP (绿光)-LC3B 串联荧光蛋白可用于检测自噬流水平的融合蛋白。它利用了酸性自溶酶体和中性自噬体之间的 pH 差异以及不同荧光素对 pH 的敏感性差异，以监控从自噬体到自溶酶体的进程 (图 6a)。图 6. 串联荧光探针及荧光共定位检测自噬流【a: mCherry-eGFP-LC3B 串联荧光探针示意图与机制 [6]；b: p27+/+ 和p27-/- … Web15 sep. 2024 · Main methods: The effects of M 4 IDP on proliferation, cell cycle and ROS production were determined by CCK-8 and flow cytometry assays. Annexin-V-FITC/PI, Hoechst 33258, MDC staining assays and Ad-mCherry-GFP-LC3B fluorescence assay were performed to investigate apoptosis and autophagy.

Web摘要: 目的探讨顺铂对HeLa人宫颈癌细胞中Nrf2蛋白表达及核转位的影响，并观察细胞自噬和凋亡的变化。方法使用不同浓度的顺铂(0、2.5、5、10 μmol/L)干预HeLa人宫颈癌细胞，MTT法检测细胞活性，Transwell实验观察细胞迁移能力，mCherry-GFP-LC3B融合蛋白腺病毒转染HeLa细胞观察自噬水平的变化，流式细胞术 ... http://www.bioon.com.cn/news/showarticle.asp?newsid=92825

Web3 apr. 2024 · Virus particles were collected after 48 h infection, filtered through a 0.22 μm membrane, added to HCT116 cells, and then incubated for 24 h at 37 °C. The medium was replaced and HCT116 cells were selected for puromycin tolerance (5 μg/mL) to obtain Caspase 3 knock-down, GFP-LC3B, or mcherry-EGFP-LC3B overexpression cells. Web4 jan. 2024 · Like CQ treatment, an accumulation of LC3 puncta with strong expression of both GFP and mCherry was observed in high glucose treated cells (Supplementary Fig. 2d).

Webmcherry-GFP-Lc3 reporter cells enable flow cytometric quantification and sorting of cells based on autophagic flux. (A) Quantification of autophagic flux by flow cytometry using …

WebGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统. 自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点 … feed the world lyrics and chordsWebKEYWORDS pBABE-puro mCherry-EGFP-LC3B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6952) AUTHORS N'Diaye EN, Kajihara KK, Hsieh I, Morisaki H, Debnath J, Brown EJ TITLE PLIC proteins or ubiquilins regulate autophagy-dependent cell survival during nutrient starvation. feed the world banksyWeb8 jul. 2024 · Inmortalized Human Chondrocytes (T/C28a2) were employed to develop an autophagy reporter cell line using LC3 as a fluorescent reporter (mCherry-EGFP-LC3B) for autophagy. Figure 1 shows the schematic workflow of the screening assay starting with the development of the autophagy reporter cell line in human chondrocytes (mCherry-EGFP … feed the world charityWeb14 apr. 2024 · Stable MCF10A cell lines carrying pBABE-puro-mCherry-EGFP-LC3B were established as described previously in the section of generation of stable cell lines. Cells were imaged using a Zeiss LSM 780 microscope with a 63X oil immersion objective lens, as described previously in the confocal section. mCherry and EGFP signals were analyzed … feed the world band airWebof the GFP fluorescent signal to acidic conditions of the lysosome lumen, leading to its inactivation compared with the mCherry fluorescence, which is more stable. Thus, colocalization of GFP and mCherry fluorescence (yellow puncta) indicates that the tandem protein is not localized in compartments fused with a lysosome (i.e., on the phagophore or feed the world let them knowWebmCherry-GFP-LC3B慢病毒 mCherry-GFP-LC3B腺相关病毒四．自噬双标系统的工作原理未发生自噬的细胞及含有自噬体的细胞中，由于mCherry与GFP共同表达，细胞呈现黄色荧光。当自噬体与溶酶体融合形成自噬溶酶体后，酸性的溶酶体环境使酸敏感的GFP荧光淬灭，而mCherry不 ... feed the world lyrics youtubeWeb21 jan. 2024 · HepG2 cells were transfected with a adenovirus expressing mCherry-GFP-LC3B fusion protein (Ad-mCherry-GFP-LC3B) (C3011, Beyotime Bio, China) according to the manufacturer’s instructions. After adenovirus transfection at a multiplicity of infection (MOI) of 20 for 24 h, the adenovirus-containing culture medium was removed and then … feed the world let them know it\u0027s christmas