Tg mbp:mcherry-caax

Author: pnaq

August undefined, 2024

WebFor your Materials & Methods section: mCherry-CaaX (Hras) was a gift from Rob Parton (Addgene plasmid # 108886 ; http://n2t.net/addgene:108886 ; RRID:Addgene_108886) For … Web50 rows · Tg (mbp:EGFP-CAAX) ID ZDB-TGCONSTRCT-120103-3 Name Tg (mbp:EGFP-CAAX) Previous Names None Type engineered_plasmid Regulatory Regions mbpa ( 1) …

Regulation of developing myelin sheath elongation by

WebOur analyses of myelination using Tg(mbp:EGFP-CAAX) indicated that despite the extensive myelination of cell bodies, ... (A and B) Confocal images of single oligodendrocytes labeled mosaically using mbp:mCherry-CAAX in wildtype (A) and caspr sa12772 mutants at 5 dpf. Scale bar, 10 μm. WebTg (smyhc1:mCherry-CAAX) This construct is targeted to the plasma membrane by the CAAX box. The CAAX box (KLNPPDESGPGCMSCKCVLS.) encodes a 20 amino acid fusion … blood on pads for women

ZFIN Transgenic Construct: Tg(mpeg1:mCherry-CAAX)

Web6 Apr 2015 · To visualize myelination in live animals, we used the Tg(mbp:mCherry-CAAX) and Tg(mbp:EGFP-CAAX) fish lines, and found that, although the onset of myelination was … Web24 Jun 2013 · In order to test this directly, we analyzed individual axons in wild-type and Tg (mbp:ca-Fyn) animals and found that individual myelinated axons in Tg (mbp:ca-Fyn) were covered by about 69% more myelin along their length compared to controls by 5 dpf (wild-type 35% ± 20%, 13 axons in nine animals; Tg (mbp:ca-Fyn) 59% ± 25, ten axons in ten … WebpEGFP-C1 (Search Vector Database) Backbone manufacturer Clontech Backbone size w/o insert (bp) 4700 Total vector size (bp) 4731 Vector type Mammalian Expression … free crossword puzzles us

Regeneration of myelin sheaths of normal length and thickness in …

Addgene: PhyB-mCherry-CAAX

WebThe red-fluorescing tg(sox10:mRFP) and tg(mbp:mCherry) lines were used to visualise oligodendrocyte structure early and late in development, respectively, and the green … Web5 Apr 2024 · We previously developed expression plasmids encoding GFP-binding peptide (or nanobody) fused to APEX, which allows EM localisation of GFP-tagged proteins in vivo. Here, we have generated conditionally stable GFP- and … blood on puppy toysWebDownload scientific diagram mbp + membrane changes are observed following exposure to lysolecithin. All images are lateral views of the spinal cord with anterior to the left and … free crossword puzzles wa

"Web25 May 2024 · The Tg (mbp:mCherry-NTR) and Tg (mbp:nls-GFP) lines used were generated as part of this study. Generation of the mbp:mCherry-NTR and mbp:nls-GFP transgenic lines We used the tol2kit [ 63] to construct the plasmids required create the Tg (mbp:mCherry-NTR) and Tg (mbp:nls-GFP lines. " - Tg mbp:mcherry-caax

Tg mbp:mcherry-caax

Characterization of OPCs in the zebrafish spinal cord a, Confocal …

Web5 May 2016 · The transgenic reporter Tg(mbp:mCherry-CAAX) was generated as part of this investigation. Generation of Fluorescent Fusion Proteins to Visualize Myelination along Single Axons. Full-length cntn1a cDNA was amplified by RT-PCR from 3 dpf total RNA using primers 1 (EcoRI-Kozak-cntn1a-F, ... http://sequenceontology.org/browser/current_svn/term/SO:0000637

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Web22 Oct 2016 · To visualize myelination in vivo, we used the plasmid (mbp-EGFP-CAAX) to label single mature oligodendrocytes in the ventral spinal cord, and observed a roughly 30 % increase in the myelin sheath number of individual oligodendrocytes in PTZ-treated animals (10.12 ± 0.56 control vs. 13.11 ± 0.54 PTZ) (Fig. 1 c, d). WebDownload scientific diagram Characterization of OPCs in the zebrafish spinal cord a, Confocal image of a Tg(olig1:memEYFP),Tg(olig1:nls-mApple) zebrafish at the level of …

Web11 Dec 2024 · The following transgenic zebrafish lines were used: tg(sox10:mRFP) vu234 (ref. 21, kindly provided by D. Lyons, Edinburgh) and two lines generated in-house, … WebPlasmid PhyB-mCherry-CAAX from Dr. Chao Tang's lab contains the insert PhyB and is published in Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub …

Web22 Oct 2016 · AB/WT and Tg (mbp:mCherry-CAAX) (gifted from David A Lyons, University of Edinburgh, Edinburgh, UK) zebrafish strains were used in this study. Zebrafish embryos … WebThat Utilize Tg(mpeg1.1:mCherry-CAAX) Construct . Genomic Feature Affected Genomic Regions sh378Tg: Transgenics . That Utilize Tg(mpeg1.1:mCherry-CAAX) Construct . Fish …

WebThat Utilize Tg(mpeg1:mCherry-CAAX) Construct . Fish Affected Genomic Regions Phenotype Gene Expression; gl26Tg: zbtb11 gl11/gl11; gl26Tg: zbtb11; Fig. 3 from …

blood on pillow lung cancerWebengineered_region ( SO:0000804) plasmid ( SO:0000155) Children: promoter_trap_construct ( SO:0001478) engineered_episome ( SO:0000779) enhancer_trap_construct ( SO:0001479) gene_trap_construct ( … free crossword puzzles usa today crosswordWeb( K ) Images show binding of MFG-E8-EGFP (cyan) to myelin sheaths or fragments (magenta) in Tg(mbp:mCherry-CAAX) larvae. Images are masked by the 552nm-channel. … free crossword puzzles uk daily mailWeb6 Apr 2015 · Images of individual cells in the figure panels are labelled by mbp:mCherry-CAAX, while images of stable transgenic lines are labelled by the “Tg” designation … free crossword puzzles washington poWebINVESTIGATION Whole Genome Sequencing-Based Mapping and Candidate Identiﬁcation of Mutations from Fixed Zebraﬁsh Tissue Nicholas E. Sanchez,*, 1Breanne L. Harty,*, ,2 Thomas O’Reilly-Pol,† Sarah D. Ackerman,*,3 Amy L. Herbert,* Melanie Holmgren,* Stephen L. Johnson,† Ryan S. Gray,*,4 and Kelly R. Monk*,2 5 *Department of Developmental … blood on q tip after cleaning earWebMultiple system atrophy (MSA) is a debilitating movement disorder with unknown etiology. Patients present characteristic parkinsonism and/or cerebellar dysfunction in the clinical phase, resulting... blood on qtip after cleaning earsWebTg (mbp:mCherry-CAAX) ( 1) Type engineered_plasmid Regulatory Regions mbpa ( 1) Coding Sequences CAAX ( 1) mCherry ( 1) [Em λ] [Ex λ] 610 (red) 587 (red) … blood on q-tip after cleaning ears